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rabbit anti human her4 erbb4  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti human her4 erbb4
    Rabbit Anti Human Her4 Erbb4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+her4+erbb4/Phospho-HER4%2FErbB4+(Tyr1284)%2FEGFR+(Tyr1173)+Rabbit+mAb/pmc06247692-141-44-51
    Average 95 stars, based on 124 article reviews
    rabbit anti human her4 erbb4 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Saline:

    Article Title: HER4 expression in estrogen receptor-positive breast cancer is associated with decreased sensitivity to tamoxifen treatment and reduced overall survival of postmenopausal women
    Article Snippet: After calculating the protein concentration with the Pierce BCA protein assay kit (ThermoFisher), 25 μg total protein per lane were separated in 7.5% SDS-PAGE under reducing conditions (mercaptoethanol) and blotted onto polyvinylidene difluoride (PVDF) membranes. .. Membranes were blocked with Tris-buffered saline supplemented with 5% low-fat milk and 2% Tween for 2 h and then incubated overnight at 4 °C using the following primary antibodies: rabbit anti-human HER1 (D38B1; 1:1000), rabbit anti-human HER2 (29D8; 1:4000), rabbit anti-human HER3 (D22C5; 1:200), rabbit anti-human HER4/ErbB4 (111B2; 1:1000) (all from Cell Signaling Technology), rabbit anti-human β-actin (A2066; 1:5000, Sigma-Aldrich Chemie GmbH), and mouse anti-estrogen receptor (NCL-L-ER-6F11; 1:500, Leica Microsystems). .. The next day, after washing the membrane, incubation with secondary antibodies (goat anti-rabbit 7074 HRP-conjugated and horse anti-mouse (7076) HRP-conjugated; both 1:2000, and both from Cell Signaling Technology) was performed for 1 h at room temperature.

    Incubation:

    Article Title: HER4 expression in estrogen receptor-positive breast cancer is associated with decreased sensitivity to tamoxifen treatment and reduced overall survival of postmenopausal women
    Article Snippet: After calculating the protein concentration with the Pierce BCA protein assay kit (ThermoFisher), 25 μg total protein per lane were separated in 7.5% SDS-PAGE under reducing conditions (mercaptoethanol) and blotted onto polyvinylidene difluoride (PVDF) membranes. .. Membranes were blocked with Tris-buffered saline supplemented with 5% low-fat milk and 2% Tween for 2 h and then incubated overnight at 4 °C using the following primary antibodies: rabbit anti-human HER1 (D38B1; 1:1000), rabbit anti-human HER2 (29D8; 1:4000), rabbit anti-human HER3 (D22C5; 1:200), rabbit anti-human HER4/ErbB4 (111B2; 1:1000) (all from Cell Signaling Technology), rabbit anti-human β-actin (A2066; 1:5000, Sigma-Aldrich Chemie GmbH), and mouse anti-estrogen receptor (NCL-L-ER-6F11; 1:500, Leica Microsystems). .. The next day, after washing the membrane, incubation with secondary antibodies (goat anti-rabbit 7074 HRP-conjugated and horse anti-mouse (7076) HRP-conjugated; both 1:2000, and both from Cell Signaling Technology) was performed for 1 h at room temperature.



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